BRCA1,2-DIAGNOSTICS
BRCA1,2-DIAGNOSTICS
The gene expression kit is designed to detect 16 germline mutations in the BRCA1 and BRCA2 genes and is used in the diagnosis of hereditary forms of breast, ovarian, pancreatic, and stomach cancer in order to determine an effective treatment strategy.
Advantages
- Beside the 8 most common mutations, the kit identifies 8 additional mutations, widely spread in Eurasia.*
*Information on the frequency of hereditary mutations in the genes of the DNA repair system (homologous recombination repair, HRR) is taken from the oncoBRCA database, obtained as part of the “Hereditary Syndromes in the Russian Federation” project, https://oncobrca.ru/
Detection method
Real-time PCR (melting profile)
Analysis type
Qualitative
Mutations detection
16
Samples
Peripheral blood, buccal scraping
Analytical sensitivity
10 copies of the BRCA1, BRCA2 genes per 1 µl of the DNA solution
Sensirtivity
100 % (with confidence interval of 95 %)
Specificity
100 % (with confidence interval of 95 %)
Time per 1 analysis
90 minutes
Number of reactions
48
Equipment
CFX96, DTPrime, Rotor-Gene Q, QuantStudio 5
Transportation and
Storage conditions
Storage conditions
-16 …-24 °C 12 months
+2 …+8 °C 30 days
+15 …+25 °C 5 months
+2 …+8 °C 30 days
+15 …+25 °C 5 months
Designed by