Scientists from the Russian company TestGene have developed a test system for determination of the effective concentration of fragmented DNA

The "Quasar Red" kit is designed to assess the concentration and quality of human genomic DNA and makes it possible to determine the suitability of the sample for subsequent NGS diagnostics. Samples with inhibitors or with a low effective concentration of DNA are detected as a result of the analysis. The test is carried out by the method of multiplex quantitative PCR.

The use of the "Quasar Red" test system is especially important when working with DNA isolated from histological paraffin blocks, as it increases the reproducibility and reliability of the target NGS sample preparation and reduces the risk of obtaining false results.

"The use of the advanced enzymatic system, increased intensity of the signal makes the "Quasar Red" kit developed by us one of the best offers on the market. One of the advantages of our kit is simultaneous determination of two DNA fragments of different lengths in one test tube, which makes it possible to assess the degree of fragmentation within a single reaction. And the separate channel to the internal control sample detects the presence of inhibitors in the sample," says Gleb Speshilov, one of the developers of the test system.

Preliminary assessment of DNA quality before sample preparation for NGS analysis helps to obtain more accurate results of sequencing.

This is especially essential if the biomaterial samples were stored for a long time without fixation and partial DNA degradation could occur.
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