Testgene - BRCA1,2-TISSUE

BRCA1,2-TISSUE

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PCR

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BRCA1,2-TISSUE

The gene expression kit is designed to detect 16 somatic mutations in the BRCA1 and BRCA2 genes and is used in the diagnosis of hereditary forms of breast, ovarian, pancreatic, and stomach cancer. The test allows predicting the course of the disease and determining an effective treatment strategy with both targeted medication and various chemotherapy programs.

Advantages

Beside the 8 most common mutations, the kit identifies 8 additional mutations, widely spread in Eurasia*.

*Information on the frequency of hereditary mutations in the genes of the DNA repair system (homologous recombination repair, HRR) is taken from the oncoBRCA database, obtained as part of the “Hereditary Syndromes in the Russian Federation” project, https://oncobrca.ru/

Detection method

Real-time PCR (melting profile)

Analysis type

Qualitative

Mutations detection

BRCA1

c.5266dupC, c.181T>G, c.5251C>T, c.5161C>T, c.4035delA, c.1961delA, c.4675G>A, c.68_69del, c.3700_3704del, c.4689C>G, c.3756_3759de

BRCA2

c.3749dupA, c.961_962insAA, c.2897_2898del, c.8754+1G>A, 6174delT

Samples

Formalin-fixed and paraffin-embedded tissue (FFPE blocks)

Analytical sensitivity

10 copies of the BRCA1, BRCA2 genes per 1 µl of the DNA solution

Sensirtivity

100 % (with confidence interval of 95 %)

Specificity

100 % (with confidence interval of 95 %)

Time per 1 analysis

90 minutes

Number of reactions

Equipment

CFX96, DTPrime, Rotor-Gene Q, QuantStudio 5

Transportation and
Storage conditions

-16 …-24 °C 12 months
+ 2 …+8 °C 30 days
+15 …+25 °C 5 days

SUBMIT AND APPLICATION

BRCA1,2-TISSUE

BRCA1,2-TISSUE

Advantages

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