TEST-BRAF-KRAS-TISSUE
PCR
TEST-BRAF-KRAS-TISSUE
The reagent kit is designed for determination of BRAF and KRAS genes mutation status and is used for patients examination diagnosed with tumor diseases, mainly lung cancer, in order to determine an effective strategy for targeted therapy, predict or monitor the response to cancer therapy and the risk of recurrence or metastasis, regardless of the disease stage in all population gropus.
Advantages
- Fast amplification protocol — 60-65 minutes
- The method is more sensitive and specific to determine an effective strategy of targeted therapy
- Test-BRAF-KRAS-tissue allows to diagnose mutations -V600E , V600Ec* in the BRAF gene codon 600, G12C in the KRAS gene
Detection method
PCR-RT
Analysis type
Qualitative
Количество мутаций
V600E, V600E complex
Mutations in the BRAF gene (detects mutations, but does not differentiate them)
G12C
mutation in the KRAS gene
Samples
Human genomic DNA samples isolated from tissue
Analytical sensitivity
76-100 copies per 1 µl
Sensirtivity
100 %
Specificity
100 %
Time per 1 analysis
60-65 min.
Number of reactions
28
Equipment
CFX96, DTprime, Rotor-Gene Q, QuantStudio 5, FLUORITE
Number of isolations
24
Format
Liquid
Transportation and
Storage conditions
Storage conditions
- 16 ...- 24°С 12 months
+2 ...+8°С 1 hour
+2 ...+8°С 1 hour
Designed by