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TEST-NRAS-TISSUE

TEST-NRAS-TISSUE

The reagent kit is used in the examination of patients diagnosed with colorectal cancer to determine indications for targeted therapy.

Advantages

  • Detects 8 mutations of the NRAS gene.

Reagent kits cannot be used to diagnose any pathology and are intended only for qualitative determination of the NRAS gene mutation status to determine indications for targeted therapy.

Detection method
Real-time PCR
Analysis type
Qualitative
Mutations detection
8
12 codon
Gly12Asp, Gly12Cys, Gly12Ser
13 codon
Gly13Asp, Gly13Arg
61 codon
Gln61Lys, Gln61Leu, Gln61Arg
Samples
Formalin-fixed and paraffin-embedded tissue (FFPE blocks)
Analytical sensitivity
10 copies of the NRAS gene per 1 µl of the DNA solution
Sensirtivity
94.1 % (with confidence interval of 90 %)
Specificity
89.1 % (with confidence interval of 90 %)
Time per 1 analysis
60-80 minutes
Equipment
CFX96, DTPrime, Rotor-Gene Q, QuantStudio 5, LC96, Abbot m2000rt
Number of isolations
5 / 24
Transportation and
Storage conditions
+ 2 …+8 °C 12 months
+15 …+25 °C 5 days
More

SUBMIT AND APPLICATION

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