Reagent kits cannot be used to diagnose any pathology and are intended only for qualitative determination of the NRAS gene mutation status to determine indications for targeted therapy.
Advantages
Detects 8 mutations of the NRAS gene.
Detection method
Real-time PCR
Analysis type
Qualitative
Mutations detection
8
12 codon
Gly12Asp, Gly12Cys, Gly12Ser
13 codon
Gly13Asp, Gly13Arg
61 codon
Gln61Lys, Gln61Leu, Gln61Arg
Samples
Formalin-fixed and paraffin-embedded tissue (FFPE blocks)
Analytical sensitivity
10 copies of the NRAS gene per 1 µl of the DNA solution
Sensirtivity
94.1 % (with confidence interval of 90 %)
Specificity
89.1 % (with confidence interval of 90 %)
Time per 1 analysis
60-80 minutes
Equipment
CFX96, DTPrime, Rotor-Gene Q, QuantStudio 5, LC96, Abbot m2000rt
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