MTB-RESIST-I-TEST
PCR
MTB-RESIST-I-TEST
The kit is designed for detection of mutations associated with drug resistance in the tuberculosis complex mycobacteria DNA with determination of sensitivity to the first-line chemotherapy drugs and their analogues. The result of the assay allows for choosing of an appropriate therapy for patients with the confirmed diagnosis of pulmonary and extrapulmonary tuberculosis.
Advantages
- The resistance of the tuberculosis complex mycobacteria to rifampicin (rpoB), isoniazid (katG and inhA) and their analogues is determined.
- Three test tubes per patient.
- The IC sample is included.
- Fast result.
Detection method
Real-time PCR (melting curves)
Analysis type
Qualitative
Targets
rpoB
polymorphisms of codons 510–533, D516V, D516Y, codon 526, L533R, L533P, S531L
katG
S315T, S315N, S315R, S315I polymorphisms
inhA
C-15T polymorphism, and polymorphisms of the region -20 – +6 are also detected, but not differentiated
Samples
Sputum, bronchoalveolar lavage, bronchial washing, gastric washing, pleural fluid, blood, urine, microbiological cultures, prostate secretion, tissue (biopsy and surgical) material, synovial fluid, pericardial fluid and cerebrospinal fluid
Analytical sensitivity
at least 5000 copies of genomic DNA per 1 ml of biomaterial
Sensirtivity
100 %
Specificity
100 %
Time per 1 analysis
125-165 min.
Number of reactions
96
Equipment
CFX96, DTprime, Rotor-Gene Q, QuantStudio 5
Transportation and
Storage conditions
Storage conditions
-18…-22 °C 12 months
+2 …+8 °C up to 5 days
freezing / thawing is allowed up to 5 times
+2 …+8 °C up to 5 days
freezing / thawing is allowed up to 5 times
Designed by