MTB-TEST
PCR
MTB-TEST
The kit is designed for detection of the tuberculosis and non-tuberculosis complex mycobacteria and their differentiation in patients with suspected pulmonary and extrapulmonary tuberculosis, mycobacteriosis.
Advantages
- One test tube per patient.
- Detection of the tuberculosis complex mycobacteria using two targets simultaneously, both targets are recorded via one channel (FAM).
- The IC sample is included.
- Fast result.
- The risk of contamination is reduced due to the inclusion of uracil-DNA-glycosylase (UDG) and 2'-deoxyuridine-5'-triphosphate (dUTP) in the reaction mix.
Detection method
Real-time PCR
Analysis type
Qualitative
Detection and differentiation
Mycobacterium tuberculosis complex
М.tuberculosis, M.bovis, M.bovis BCG, M.africanum, M.canettii, M.caprae, M.microti
Nontuberculous mycobacteria
M.avium, M.abscessus, M.septicum, M.fortuitum, M.gordonae, M.intracellulare, M.kansasii, M.marinum, M.smegmatis, M.xenopi, M.ulcerans, M.terrae
Mycolicibacterium spp.
Mycobacteroides spp.
Samples
Sputum, bronchoalveolar lavage, bronchial washing, gastric washing, pleural fluid, blood,
urine, microbiological cultures, prostate secretion, tissue (biopsy and surgical) material,
synovial fluid, pericardial fluid and cerebrospinal fluid, washings from environmental objects
Analytical sensitivity
from 100 copies/ml
Sensirtivity
100 %
Specificity
100 %
Time per 1 analysis
90-110 min.
Number of reactions
96
Equipment
CFX96, DTprime, Rotor-Gene Q, QuantStudio 5
Transportation and
Storage conditions
Storage conditions
-18 …-22 °C 12 months
+2 …+8 °C up to 30 days
+15 …+25 °C up to 5 days
freezing / thawing is allowed up to 10 times
+2 …+8 °C up to 30 days
+15 …+25 °C up to 5 days
freezing / thawing is allowed up to 10 times
Designed by