MTB-RESIST-II-TEST
MTB-RESIST-II-TEST
The kit is designed to detect polymorphisms associated with drug resistance to second-line chemotherapy drugs (aminoglycosides and fluoroquinolones) and their analogues.
Advantages
- The resistance of the Mycobacterium tuberculosis complex to aminoglycosides (rrs and eis gene polymorphisms) and fluoroquinolones (gyrA and gyrB gene polymorphisms) and their analogs is detected.
- The IC is available.
Detection method
Real-time PCR (melting curves)
Analysis type
Qualitative
Targets
rrs
1401A>G, 1402C>T и 1484G>T
eis
C-14G, C-14T, C-12T, G-10C, G-10A и G-37T
gyrA
p.G88C, p.A90V, p.S91P, p.D94G, p.D94N, p.D94H, p.D94A и p.D94Y
gyrB
p.D461H, p.D461N, p.N499D, p.E501V и p.A504V
Samples
Sputum, bronchoalveolar lavage, bronchial lavage, gastric lavage, pleural fluid, blood, urine, cultures of microorganisms, prostate secretion, tissue (biopsy and surgical) material, synovial fluid, pericardial fluid and cerebrospinal fluid
Analytical sensitivity
at least 2000 copies of genomic DNA per 1 ml of biomaterial
Sensirtivity
100 %
Specificity
100 %
Time per 1 analysis
120-165 min.
Number of reactions
12 / 96
Equipment
CFX 96, DTprime, Rotor-Gene Q, QuantStudio 5
Transportation and
Storage conditions
Storage conditions
-18…-22 °C 12 months
+2…+8 °C up to 30 days
freezing / thawing is allowed up to 5 times
+2…+8 °C up to 30 days
freezing / thawing is allowed up to 5 times
Designed by